VACCINATION
Puppies receive antibiodies that protect them from parvovirus These antibodies are transferred from the mother to the puppies in the first 3 days of life. Mother's first milk, colostrum, is the source of these antibodies.
How long the protection from mother will last is uncertain. Some pups are no longer protected by mother as early as 6 weeks. Some pups are protected by mother for 12 weeks.
At 6 weeks of age about half of puppies are no longer protected by "mom". It is important that they be vaccinated so that they will be protected from Parvo. However, if "mom" is still protecting the pup, the vaccination will be ineffective. "First shots", "Temporary shots", "Puppy shots" are terms sometimes used to define this undetermined vaccine status.
At 9 weeks of age, 8 out of 10 pups need the vaccine and will respond to vaccination. Alas, 2 out of 10 are still protected by maternal antibodies they received from "mom" 8 1/2 weeks earlier, and the shot will not work.
12 weeks of age represents the time when vaccine should work for nearly all puppies. A booster in 3 weeks is a good idea.
In summary: Vaccination prior to 12 weeks is a wise. Two doses after 12 weeks of age is essential to insure a good immune response to vaccination.